GLUE Installation on Ubuntu

GLUE for virus sequence data, with a focus on variation, evolution and sequence interpretation.

Installation on Ubuntu

http://tools.glue.cvr.ac.uk/#/installation

Install Java

sudo add-apt-repository ppa:webupd8team/java
sudo apt update
sudo apt install oracle-java8-set-default
java -version

Install MySQL

sudo apt install mysql-server
sudo mysql
create user 'gluetools'@'localhost' identified by 'glue12345';
create database GLUE_TOOLS character set UTF8;
grant all privileges on GLUE_TOOLS.* to 'gluetools'@'localhost';
exit

Test DB – login with user gluetools

mysql -u gluetools --password=glue12345

Install GLUE

Download the GLUE install zip from the GLUE download page. Unzip the GLUE install zip file in a convenient location (e.g. /home/fred).

wget http://tools.glue.cvr.ac.uk/downloads/glueInstallDir-1.1.6.zip

Insatll unzip

sudo apt install unzip

Unzip

unzip glueInstallDir-1.1.6.zip

Add GLUE_HOME to your bash path

Edit ~$.bashrc with

nano /home/user/.bashrc
export GLUE_HOME=/home/user/gluetools
export PATH=${PATH}:${GLUE_HOME}/bin

Restart terminal for paths to take effect.

Make sure the bash script is executable:

chmod u+x /home/user/gluetools/bin/gluetools.sh

Download GLUE Engine

Download the GLUE engine jar file from the GLUE download page. Place it inside the gluetools/lib directory.

cd /home/user/gluetools/lib
wget http://tools.glue.cvr.ac.uk/downloads/gluetools-core-1.1.6.jar

Edit GLUE Config

In a text editor, load the XML file gluetools/conf/gluetools-config.xml. You will need to make sure that the database section specifies the correct MySQL username, password and database name as necessary, by editing the contents of the username, password and jdbcUrl elements.

nano /home/user/gluetools/conf/gluetools-config.xml

	
		gluetools
		glue12345
		MySQL
		jdbc:mysql://localhost:3306/GLUE_TOOLS?characterEncoding=UTF-8	
	
	
	

Running the GLUE command line

gluetools.sh

Exit GLUE

exit

Software integration: BLAST+

Download and install BLAST+ 2.2.31 from NCBI’s BLAST+ FTP page.

wget ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.2.31/ncbi-blast-2.2.31+-x64-linux.tar.gz
tar xvzf ncbi-blast-2.2.31+-x64-linux.tar.gz

To integrate BLAST+ into GLUE, load the XML file gluetools/conf/gluetools-config.xml in a text editor.


	
	
	
		
		
			gluetools.core.programs.blast.blastn.executable
			/home/user/ncbi-blast-2.2.31+/bin/blastn
		
		
			gluetools.core.programs.blast.tblastn.executable
			/home/user/ncbi-blast-2.2.31+/bin/tblastn
		
		
			gluetools.core.programs.blast.makeblastdb.executable
			/home/user/ncbi-blast-2.2.31+/bin/makeblastdb
		
		
			gluetools.core.programs.blast.temp.dir
			/home/user/gluetools/tmp/blastfiles
		
		
			gluetools.core.programs.blast.db.dir
			/home/user/gluetools/tmp/blastdbs
		
		
			gluetools.core.programs.blast.search.threads
			4
		
	
	

Software integration: MAFFT

Download MAFFT from the CBRC MAFFT page and install it locally.

Download https://mafft.cbrc.jp/alignment/software/linux.html

wget https://mafft.cbrc.jp/alignment/software/mafft_7.407-1_amd64.deb
sudo dpkg -i mafft_x.xxx-x_amd64.deb

	
	
	
		
		
			gluetools.core.programs.mafft.executable
			/usr/bin/mafft
		
		
			gluetools.core.programs.mafft.cpus
			4
		
		
			gluetools.core.programs.mafft.temp.dir
			/home/user/gluetools/tmp/mafftfiles
		
	
	

Software integration: RAxML

GLUE uses RAxML as part of its maximum likelihood genotyping procedure, and for general phylogenetics.

We suggest RAxML be compiled locally so that it is optimised for your hardware. Instructions can be found at the Exelixis Lab RAxML page.

git clone https://github.com/stamatak/standard-RAxML.git
cd standard-RAxML
make -f Makefile.AVX.gcc

This will generate a RAxML executable called raxmlHPC-AVX.

Add the location to the gluetools config.


	
	
	
		
		
			gluetools.core.programs.raxml.raxmlhpc.executable
			/home/user/standard-RAxML/raxmlHPC-AVX
		
		
			gluetools.core.programs.raxml.raxmlhpc.cpus
			4
		
		
			gluetools.core.programs.raxml.temp.dir
			/home/user/gluetools/tmp/raxmlfiles
		
	
	

Example project

http://tools.glue.cvr.ac.uk/#exampleProject

cd /home/user/gluetools/projects
wget http://tools.glue.cvr.ac.uk/downloads/exampleProject-1.1.6.zip
unzip exampleProject-1.1.6.zip

Command line interpreter

http://tools.glue.cvr.ac.uk/#/interactiveCommandLine

cd ~/gluetools/projects/exampleProject
gluetools.sh

In GLUE

run file exampleProject.glue

Offline HCV-GLUE

http://hcv.glue.cvr.ac.uk/#/aboutGlueProject

Download an HCV-GLUE project build

gunzip -c ncbi_hcv_glue.sql.gz | mysql --user=gluetools --password=glue12345 GLUE_TOOLS
cd ~/gluetools/projects
gluetools.sh
list project
project hcv

Example use: Combined genotyping and drug resistance analysis reports

This first example will produce a report for a FASTA file, e.g. resistanceGeno1.fasta. A preview of the JSON output is available here.

gluetools.sh -p cmd-result-format:json -EC -i project hcv module hcvDRReporter invoke-function reportFasta resistanceGeno1.fasta
19:00:23.978 NashornJsScript WARNING: The convertTableToObjects option is deprecated. Wrap the call to glue.command(...) with glue.tableToObjects(...) instead.
19:01:56.434 NashornJsScript WARNING: The convertTableToObjects option is deprecated. Wrap the call to glue.command(...) with glue.tableToObjects(...) instead.
19:01:56.467 NashornJsScript WARNING: The convertTableToObjects option is deprecated. Wrap the call to glue.command(...) with glue.tableToObjects(...) instead.
19:01:56.649 NashornJsScript WARNING: The convertTableToObjects option is deprecated. Wrap the call to glue.command(...) with glue.tableToObjects(...) instead.
Exception at line 1 of inline-command
Error: Required property "showMatchesAsTable" missing from element "scan"
	at recastException (:75)
	at command (:126)
	at  (hcvDRReporter.js:39)
	at forEach (:161)
	at  (hcvDRReporter.js:38)
	at inMode (:133)
	at  (hcvDRReporter.js:37)
	at forEach (:161)
	at reportFasta (hcvDRReporter.js:26) 
Cause: Required property "showMatchesAsTable" missing from element "scan"

This example will produce a report for a SAM/BAM file, e.g. ngsData.bam. A preview of the JSON output is available here.

gluetools.sh -p cmd-result-format:json -EC -i project hcv module hcvDRReporter invoke-function reportBam ngsData.bam

{
    "bamReport":{
        "bamFilePath":"ngsData.bam",
        "samReferenceResults":[
            {
                "id":"RS15005637-A_S51.consensus1",
                "samReference":{
                    "name":"RS15005637-A_S51.consensus1",
                    "length":9266,
                    "index":0,
                    "species":null,
                    "assembly":null
                },
                "isForwardHcv":true,
                "isReverseHcv":false,
                "genotypingResult":{
                    "queryName":"RS15005637-A_S51.consensus1",
                    "genotypeFinalClade":"AL_1",
                    "genotypeCladeBalance":"AL_1:100.00%",
                    "genotypeClosestMemberAlignmentName":"AL_1a",
                    "genotypeClosestMemberSourceName":"ncbi-refseqs",
                    "genotypeClosestMemberSequenceID":"HQ850279",
                    "genotypeClosestTargetAlignmentName":"AL_1a",
                    "genotypeClosestTargetSourceName":"ncbi-refseqs",
                    "genotypeClosestTargetSequenceID":"HQ850279",
                    "subtypeFinalClade":"AL_1a",
                    "subtypeCladeBalance":"AL_1a:100.00%",
                    "subtypeClosestMemberAlignmentName":"AL_1a",
                    "subtypeClosestMemberSourceName":"ncbi-refseqs",
                    "subtypeClosestMemberSequenceID":"HQ850279",
                    "subtypeClosestTargetAlignmentName":"AL_1a",
                    "subtypeClosestTargetSourceName":"ncbi-refseqs",
                    "subtypeClosestTargetSequenceID":"HQ850279"
                },
                "licensedDrugRegimens":[
                    {
                        "drugRegimen":{
                            "id":"gzr_ebr",
                            "displayName":"grazoprevir/elbasvir ± ribavirin",
                            "shortDisplayName":"GZR/EBR",
                            "daaDrugsInRegimen":{
                                "drug":[
                                    {
                                        "id":"elbasvir",
                                        "categoryId":"NS5A_inhibitor",
                                        "categoryDisplayName":"NS5A inhibitor"
                                    },
                                    {
                                        "id":"grazoprevir",
                                        "categoryId":"NS3_inhibitor",
                                        "categoryDisplayName":"NS3/4A protease inhibitor"
                                    }
                                ]
                            }
                        },
                        "recommendationLevel":"available"
                    },
                    {
                        "drugRegimen":{
                            "id":"obv_ptv_r_dbv",
                            "displayName":"ombitasvir/paritaprevir/ritonavir + dasabuvir ± ribavirin",
                            "shortDisplayName":"OBV/PTV-r/DBV",
                            "daaDrugsInRegimen":{
                                "drug":[
                                    {
                                        "id":"dasabuvir",
                                        "categoryId":"NS5B_inhibitor",
                                        "categoryDisplayName":"NS5B RNA polymerase inhibitor"
                                    },
                                    {
                                        "id":"ombitasvir",
                                        "categoryId":"NS5A_inhibitor",
                                        "categoryDisplayName":"NS5A inhibitor"
                                    },
                                    {
                                        "id":"paritaprevir",
                                        "categoryId":"NS3_inhibitor",
                                        "categoryDisplayName":"NS3/4A protease inhibitor"
                                    }
                                ]
                            }
                        },
                        "recommendationLevel":"available"
                    },
                    {
                        "drugRegimen":{
                            "id":"sof_dcv",
                            "displayName":"sofosbuvir + daclatasvir ± ribavirin",
                            "shortDisplayName":"SOF/DCV",
                            "daaDrugsInRegimen":{
                                "drug":[
                                    {
                                        "id":"daclatasvir",
                                        "categoryId":"NS5A_inhibitor",
                                        "categoryDisplayName":"NS5A inhibitor"
                                    },
                                    {
                                        "id":"sofosbuvir",
                                        "categoryId":"NS5B_inhibitor",
                                        "categoryDisplayName":"NS5B RNA polymerase inhibitor"
                                    }
                                ]
                            }
                        },
                        "recommendationLevel":"available"
                    },
                    {
                        "drugRegimen":{
                            "id":"sof_ldv",
                            "displayName":"sofosbuvir/ledipasvir ± ribavirin",
                            "shortDisplayName":"SOF/LDV",
                            "daaDrugsInRegimen":{
                                "drug":[
                                    {
                                        "id":"ledipasvir",
                                        "categoryId":"NS5A_inhibitor",
                                        "categoryDisplayName":"NS5A inhibitor"
                                    },
                                    {
                                        "id":"sofosbuvir",
                                        "categoryId":"NS5B_inhibitor",
                                        "categoryDisplayName":"NS5B RNA polymerase inhibitor"
                                    }
                                ]
                            }
                        },
                        "recommendationLevel":"available"
                    },
                    {
                        "drugRegimen":{
                            "id":"sof_sim",
                            "displayName":"sofosbuvir + simeprevir ± ribavirin",
                            "shortDisplayName":"SOF/SIM",
                            "daaDrugsInRegimen":{
                                "drug":[
                                    {
                                        "id":"simeprevir",
                                        "categoryId":"NS3_inhibitor",
                                        "categoryDisplayName":"NS3/4A protease inhibitor"
                                    },
                                    {
                                        "id":"sofosbuvir",
                                        "categoryId":"NS5B_inhibitor",
                                        "categoryDisplayName":"NS5B RNA polymerase inhibitor"
                                    }
                                ]
                            }
                        },
                        "recommendationLevel":"suboptimal"
                    },
                    {
                        "drugRegimen":{
                            "id":"sof_vel",
                            "displayName":"sofosbuvir/velpatasvir ± ribavirin",
                            "shortDisplayName":"SOF/VEL",
                            "daaDrugsInRegimen":{
                                "drug":[
                                    {
                                        "id":"sofosbuvir",
                                        "categoryId":"NS5B_inhibitor",
                                        "categoryDisplayName":"NS5B RNA polymerase inhibitor"
                                    },
                                    {
                                        "id":"velpatasvir",
                                        "categoryId":"NS5A_inhibitor",
                                        "categoryDisplayName":"NS5A inhibitor"
                                    }
                                ]
                            }
                        },
                        "recommendationLevel":"available"
                    }
                ],
                "targetRefName":"REF_1a_HQ850279",
                "easlNgsScanResult":[
                    {
                        "referenceName":"REF_MASTER_NC_004102",
                        "featureName":"NS5A",
                        "variationName":"RAS_NS5A_31M",
                        "readsPresent":90,
                        "pctPresent":31.690140845070424,
                        "readsAbsent":194,
                        "pctAbsent":68.30985915492958,
                        "rasDetails":[
                            {
                                "easlOfficialGuidance":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "drugCategory":"NS5A_inhibitor",
                                    "drugCategoryDisplayName":"NS5A inhibitor",
                                    "cladeName":"AL_1a",
                                    "cladeDisplayName":"HCV Subtype 1a",
                                    "clinicalFinding":[
                                        {
                                            "clinicalStudyName":"c_edge_te",
                                            "clinicalStudyDisplayName":"C-EDGE TE",
                                            "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516351708",
                                            "publicationID":"Kwo_et_al_2017",
                                            "publication":"Gastroenterology",
                                            "publicationDisplayName":"Kwo et al., 2017",
                                            "pubLocation":"152(1):164-175",
                                            "authors":"Kwo P, Gane EJ, Peng CY, Pearlman B, Vierling JM, Serfaty L, Buti M, Shafran S, Stryszak P, Lin L, Gress J, Black S, Dutko FJ, Robertson M, Wahl J, Lupinacci L, Barr E, Haber B",
                                            "title":"Effectiveness of Elbasvir and Grazoprevir Combination, With or Without Ribavirin, for Treatment-Experienced Patients With Chronic Hepatitis C Infection",
                                            "year":2017
                                        },
                                        {
                                            "clinicalStudyName":"c_edge_tn",
                                            "clinicalStudyDisplayName":"C-EDGE TN",
                                            "webLink":"http://annals.org/aim/article/2279766/grazoprevir-elbasvir-combination-therapy-treatment-naive-cirrhotic-noncirrhotic-patients-chronic",
                                            "publicationID":"Zeuzem_et_al_2015a",
                                            "publication":"Annals of Internal Medicine",
                                            "publicationDisplayName":"Zeuzem et al., 2015a",
                                            "pubLocation":"163(1):1-13",
                                            "authors":"Zeuzem S, Ghalib R, Reddy KR, Pockros PJ, Ben Ari Z, Zhao Y, Brown DD, Wan S, DiNubile MJ, Nguyen BY, Robertson MN, Wahl J, Barr E, Butterton JR",
                                            "title":"Grazoprevir-Elbasvir Combination Therapy for Treatment-Naive Cirrhotic and Noncirrhotic Patients With Chronic Hepatitis C Virus Genotype 1, 4, or 6 Infection: A Randomized Trial",
                                            "year":2015
                                        },
                                        {
                                            "clinicalStudyName":"ion_post_hoc",
                                            "clinicalStudyDisplayName":"Post-hoc pooled ION trial data analysis",
                                            "webLink":"http://onlinelibrary.wiley.com/doi/10.1002/hep.28181/pdf",
                                            "publicationID":"Zeuzem_et_al_2015b",
                                            "publication":"Hepatology 2015",
                                            "publicationDisplayName":"Zeuzem et al., 2015b",
                                            "pubLocation":"62:254A",
                                            "authors":"Zeuzem S, Mizokami M, Pianko S, Mangia A, Han KH, Martin R, et al.",
                                            "title":"Prevalence of Pre-Treatment NS5A Resistance Associated Variants in Genotype 1 Patients Across Different Regions Using Deep Sequencing and Effect on Treatment Outcome with LDV/SOF",
                                            "year":2015
                                        }
                                    ],
                                    "drugGuideline":[
                                        {
                                            "drugGuidelineResistedDrug":"ledipasvir",
                                            "drugGuidelineSource":"EASL",
                                            "drugGuidelineText":"If reliable NS5A resistance testing is performed, treatment-experienced, DAA-naïve patients infected with genotype 1a with or without compensated cirrhosis who have NS5A RASs that confer high-level resistance to ledipasvir (M28A/G/T, Q30E/G/H/K/R, L31M/V, P32L/S, H58D, and/or Y93C/H/N/S) detected at baseline should be treated with the fixed-dose combination of sofosbuvir and ledipasvir for 12 weeks with ribavirin, whereas those without ledipasvir RASs at baseline can be treated with the fixed-dose combination of sofosbuvir and ledipasvir for 12 weeks without ribavirin (B1)."
                                        },
                                        {
                                            "drugGuidelineResistedDrug":"elbasvir",
                                            "drugGuidelineSource":"EASL",
                                            "drugGuidelineText":"If reliable NS5A resistance testing is performed, treatment-naïve and treatment-experienced patients infected with subtype 1a with or without compensated cirrhosis should receive the combination of grazoprevir and elbasvir for 16 weeks with daily weight-based ribavirin (1000 or 1200 mg in patients <75 kg or ≥75 kg, respectively) if their HCV RNA level is >800,000 IU/ml and NS5A RASs that confer resistance to elbasvir (M28A/G/T, Q30D/E/G/H/K/L/R, L31F/M/V, H58D and/or Y93C/H/N/S) are present at baseline. Patients infected with subtype 1a with or without compensated cirrhosis with an HCV RNA level ≤800,000 IU/ml and those with an HCV RNA level >800,000 IU/ml without elbasvir NS5A RASs at baseline should receive the combination of grazoprevir and elbasvir for 12 weeks without ribavirin (B1)."
                                        }
                                    ]
                                }
                            }
                        ]
                    }
                ],
                "inVitroNgsScanResult":[
                    {
                        "referenceName":"REF_MASTER_NC_004102",
                        "featureName":"NS3",
                        "variationName":"RAS_NS3_170V",
                        "readsPresent":334,
                        "pctPresent":80.28846153846153,
                        "readsAbsent":82,
                        "pctAbsent":19.71153846153846,
                        "rasDetails":[
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS3",
                                    "rasName":"RAS_NS3_170V",
                                    "rasRenderedName":"NS3:170V",
                                    "rasSubstitutions":"170V",
                                    "resistedDrug":"asunaprevir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":2,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS3",
                                    "rasName":"RAS_NS3_170V",
                                    "rasRenderedName":"NS3:170V",
                                    "rasSubstitutions":"170V",
                                    "resistedDrug":"paritaprevir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":2,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS3",
                                    "rasName":"RAS_NS3_170V",
                                    "rasRenderedName":"NS3:170V",
                                    "rasSubstitutions":"170V",
                                    "resistedDrug":"simeprevir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":2,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            }
                        ]
                    },
                    {
                        "referenceName":"REF_MASTER_NC_004102",
                        "featureName":"NS5A",
                        "variationName":"RAS_NS5A_31M",
                        "readsPresent":90,
                        "pctPresent":31.690140845070424,
                        "readsAbsent":194,
                        "pctAbsent":68.30985915492958,
                        "rasDetails":[
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"daclatasvir",
                                    "publication":{
                                        "id":"Lontok_et_al_2015",
                                        "displayName":"Lontok et al., 2015",
                                        "webLink":"http://onlinelibrary.wiley.com/doi/10.1002/hep.27934/abstract"
                                    },
                                    "minFoldChange":105,
                                    "maxFoldChange":105,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"daclatasvir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":100,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"daclatasvir",
                                    "publication":{
                                        "id":"Sarrazin_2016",
                                        "displayName":"Sarrazin, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0168827815006297"
                                    },
                                    "minFoldChange":100,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"elbasvir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":100,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"ledipasvir",
                                    "publication":{
                                        "id":"Lontok_et_al_2015",
                                        "displayName":"Lontok et al., 2015",
                                        "webLink":"http://onlinelibrary.wiley.com/doi/10.1002/hep.27934/abstract"
                                    },
                                    "minFoldChange":554,
                                    "maxFoldChange":554,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"ledipasvir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":100,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"ledipasvir",
                                    "publication":{
                                        "id":"Sarrazin_2016",
                                        "displayName":"Sarrazin, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0168827815006297"
                                    },
                                    "minFoldChange":100,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"ombitasvir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":100,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            },
                            {
                                "inVitroResistanceFinding":{
                                    "rasReferenceSequence":"REF_MASTER_NC_004102",
                                    "rasFeature":"NS5A",
                                    "rasName":"RAS_NS5A_31M",
                                    "rasRenderedName":"NS5A:31M",
                                    "rasSubstitutions":"31M",
                                    "resistedDrug":"velpatasvir",
                                    "publication":{
                                        "id":"Pawlotsky_2016",
                                        "displayName":"Pawlotsky, 2016",
                                        "webLink":"http://www.sciencedirect.com/science/article/pii/S0016508516300555"
                                    },
                                    "minFoldChange":100,
                                    "maxFoldChange":null,
                                    "repliconCladeId":"AL_1a",
                                    "repliconCladeDisplayName":"HCV Subtype 1a"
                                }
                            }
                        ]
                    }
                ]
            }
        ]
    }
}

Project
http://tools.glue.cvr.ac.uk/#/buildYourOwnProject